Assembly of Long-Read Sequencing

This hands-on practical workshop is an introduction to long read next-generation sequencing data and using SMRTLink.

Recommended Participants

Biologists and other life scientists planning to work with long-read (PacBio) next-generation sequencing data for genome assembly

Learning Objectives

• Perform QC evaluation and filtering on long read next-generation sequence data

• Use SMRTLink to assemble genome

• Visualise assembly using SMRTLink 

• Assess genome quality using BUSCO and Augustus 

• Export genome data files for downstream analysis

Syllabus

• Introduction to long read next-generation sequencing data

• Differences in genome assembly from short and long-read next-generation sequencing data

• Using SMRTLink to manage sample data, perform quality control filtering and for genome assembly

• Visualisation of assembled genome in SMRTLink

• Export of genome for downstream analysis and assessment of genome completeness